Learn to Pipette
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Objective:  Learn to pipette like a professional. Tips:  1. Click on objects throughout, there may be hidden information 2. Tweet any technical questions at @aminobiolab 
Welcome to the Virtual Bioengineer Simulation:
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Micropipettes are used to move small amount of liquid around accuratly. As you do more advanced experiments it becomes important to be precise in our measurements! Gather your materials and find a clean, stable surface to work on. And click (tap) on the gloves to begin! Even though you won’t be touching bacteria or reagents, it’s always best practice to do science with gloves on. You also don’t want to contaminate your pipettes with hand bacteria! 
Next
What are Pipettes?
Apply a Pipette Tip
Select a tip from the box, with the pipette vertical, push down and slightly twist to secure the pipette tip. Remember to check you have the correct size of pipette tip for the pipette you are using!
Bring the pipette to the solution you are trying to draw up. The pipette tip should be just below the surface of the solution. Click (tap) on the plunger again to release the plunger and aspirate your solution.
Next Step
Micropipettes have a volume indicator for drawing up a precise amount of fluid. Before you apply the pipette tip, adjust your volume.  There are also different pipette sizes for different amounts of volume: P1000 pipette for volumes of 100 to 1,000 uL  P100 pipette for volumes of 10 to 100 uL  P10 pipette for volumes of 0.5 to 10 uL
volume indicator Display
Great Job! Now press down on the plunger to the first stop point to draw up the volume you’ve selected on your pipette. Click (tap) on the pipette plunger. 
Bring the pipette to the destination tube and press all the way down on the plunger, past the first stop point,  to release your solution. Keep your tip just above the surface of the solution to avoid bubbles. Click (tap) the plunger again the release your solution. 
Press down on the tip ejector to remove the pipette tip over the garbage. 
Pipette-it
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Easy-Peasy! Remember to keep practicing and you’ll become a pipetting pro!
Keep learning to pipette
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Balance a centrifuge
Paint with Colourful Bacteria!
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What is DNA? 
Genetic engineering
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What is DNA?
Learn Bioplastics
Paint with Colorful bacteria
Genetic Engineering
Balance a centrifuge
Keep learning to pipette
Tip ejector collar
Plunger shaft
Volume indicator display
Hook
Plunger button
Pipette volume range
Tip cone
The tip ejector collar allows safe and easy removal of pipette tips without touching the tips by hand.
Disposable tip
Identify the parts of the micropipette. Drag each word where it should go. 
Tip ejector button
Congratulations! Those are the main parts of a micropipette.  Now, let’s see how its used it
The hook lets you rest your hand and use a lighter grip
Identify the parts of the micropipette. Drag each word where it should go.  Hint: use the back arrow if you need help
The tip cone is the area where the pipette tip gets attached
The volume indicator consists of a three-number dial and is read from top to bottom.
Micropipettes have a plunger that is depressed using the thumb and as the plunger is released, liquid is drawn into a disposable plastic tip. When the plunger is pressed again, the liquid is expelled. 
The disposable tips allow the micropipette to be used for different samples (the tip must be changed between samples)
The volume range of a pipette can significantly impact accuracy and precision. Essentially, the closer the dispense volume is to the total volume of the pipette, the better the results will be. For example, if accuracy and precision are important, users should not use a 100 μl pipette for 10 μl dispenses
Once used, tips are ejected into a disposal bin using the tip eject button that is next to the plunger.
The plunger shaft is a tube-like structure filled with air in an air displacement pipette.  As the plunger is pressed to the first stop, an internal piston displaces a volume of air equal to the volume shown on the volume indicator dial. The second stop is used only to dispense the contents of the tip.
Click on the different pipettes to learn more about it
Types of pipettes
Micropipettes are used for accurately measuring and transferring very small volumes of liquid, usually in the microliter (μL) range. Micropipettes are adjustable and come in various configurations. They have a digital or analog display to set the desired volume. They are available as single-channel or multichannel pipettes for dispensing multiple samples at once. This is a single-channel micropipette and the most popular choice in microbiology and biotechnology labs.
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Micropipettes are used for accurately measuring and transferring very small volumes of liquid, usually in the microliter (μL) range. This is a multichannel pipette. Multichannel pipettes are used for dispensing multiple samples at once. They are adjustable and in digital or analog display to set the desired volume. 
Pasteur pipettes are used for various laboratory tasks, including transferring small amounts of liquid. They were the first types of pipettes, created in the 19th century by Louis Pasteur. Traditionally made of glass, you can now also find plastic ones. They have a straight shape with a fine, tapered tip, and are often disposable.
Pipette controllers are devices that attach to a conventional glass pipette to allow a more user friendly and accurate operation. They enable the user to rapidly fill and release the pipette, using squeezable bulbs, buttons or thumb wheels.  They may be mechanical but are more commonly battery operated. Both pistol and linear designs are available.
A volumetric pipette, bulb pipette, or belly pipette allow extremely accurate measurement of the volume of a solution. It is calibrated to deliver accurately a fixed volume of liquid. These pipettes have a large bulb with a long narrow portion above with a single graduation mark as it is calibrated for a single volume (like a volumetric flask). Typical volumes are 1, 2, 5, 10, 20, 25, 50 and 100 mL. Volumetric pipettes are commonly used in analytical chemistry to make laboratory solutions from a base stock as well as to prepare solutions for titration.